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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
17/12/2010 |
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Data da última atualização: |
31/10/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, G. A. da; BERNARDI, T. L.; SCHAKER, P. D. C.; MELLO, L. M. R. de; VALENTE, P. |
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Afiliação: |
GILDO ALMEIDA DA SILVA, CNPUV; TAÍS LETÍCIA BERNARDI, UFRGS; PATRÍCIA DAYANE CARVALHO SCHAKER, UERGS; LOIVA MARIA RIBEIRO DE MELLO, CNPUV; PATRÍCIA VALENTE, UFRGS. |
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Título: |
Captan and Folpan inhibition of PCR amplification of 375 bp DNA fragment specific for the 18S region of Saccharomyces. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON CULTURE COLLECTIONS, 12., 2010, Florianópolis. Biological Resource Centrers: gateway to biodiversity and services for innovations in biotechnology: [abstracts]. [S.l]: WFCC, 2010. |
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Páginas: |
Não paginado. |
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Idioma: |
Inglês |
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Notas: |
Poster. |
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Conteúdo: |
The off-flavor metabolites produced by nutritionally fastidious yeasts can be economically disastrous to the wine producer since they render the wine unpalatable, decreasing the quality of the wine, and resulting in a lower sale price. These microorganisms are difficult to be detected by traditional culture media since these techniques involve complex enrichment. The polymerase chain reaction (PCR)is a well established molecular technique that uses tiny amounts of genetic material to be amplified, it does not depend on culture media for cell growth and so it can overcome several difficulties. This essentially enzymatic reaction can be employed to detect microorganisms in foods and beverages but it can also be susceptible to inhibitors. Molecular systematic investigation of members of the ascomycetous yeast genera or monitoring spoilage microorganisms in wine may not be an easy task due the presence of inhibitor compounds. Besides the presence of wine naturally occurring inhibitors, cultural practices, like fungicide treatments can also contribute to add external inhibiting factors if residues of these compounds are present in wine. The inhibition is likely to be dependent upon the residue levels. Captan and Folpan are widely employed in Brazilian vineyards to control fungal threats. The purpose of this work was to detect the action of these compounds upon Taq polymerase using a forward primer Fungf (5'-CTTAGTTGGTGGAGTGATTTG-3') and a reverse primer Fungr (5'-GGTACTAGCGACGGGCG-3') that amplify a 375 bp DNA fragment specific for the 18S region of fungi. The efficiency of PCR and yeast metabolism were evaluated with the strain Saccharomyces cerevisiaeEmbrapa 1vvt/97 (BRM-4687) in the presence of different concentrations of Captan (1200-recommended dose, 600, 300, 150, 75, and 37.5 ?g/mL) and Folpan (1350-recommended dose, 675, 337.5, 168.8, 84.4 and 42.2 ?g/mL). The amplification was observed only at 75, and 37.5 ?g/mL Captan. Negligible amplification was obtained at 84.4 ?g/mL Folpan and visible amplification was possible only at 42.2 ?g/mL Folpan. Captan and Folpan are, depending on the concentration, strong Taq polymerase inhibitors. The presence of both fungicides can make the hard work of monitoring process of spoilage microorganisms in wine even harder. These fungicides also showed to be strong inhibitors of the cell yeast metabolism.Key words:Fermentation, fungicides, PCR, Taq polymerase, yeast MenosThe off-flavor metabolites produced by nutritionally fastidious yeasts can be economically disastrous to the wine producer since they render the wine unpalatable, decreasing the quality of the wine, and resulting in a lower sale price. These microorganisms are difficult to be detected by traditional culture media since these techniques involve complex enrichment. The polymerase chain reaction (PCR)is a well established molecular technique that uses tiny amounts of genetic material to be amplified, it does not depend on culture media for cell growth and so it can overcome several difficulties. This essentially enzymatic reaction can be employed to detect microorganisms in foods and beverages but it can also be susceptible to inhibitors. Molecular systematic investigation of members of the ascomycetous yeast genera or monitoring spoilage microorganisms in wine may not be an easy task due the presence of inhibitor compounds. Besides the presence of wine naturally occurring inhibitors, cultural practices, like fungicide treatments can also contribute to add external inhibiting factors if residues of these compounds are present in wine. The inhibition is likely to be dependent upon the residue levels. Captan and Folpan are widely employed in Brazilian vineyards to control fungal threats. The purpose of this work was to detect the action of these compounds upon Taq polymerase using a forward primer Fungf (5'-CTTAGTTGGTGGAGTGATTTG-3') and a reverse primer Fungr (5'-GGTACTAGCGACGGGC... Mostrar Tudo |
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Palavras-Chave: |
Levedura; PCR; Polimerase. |
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Thesagro: |
Fermentação; Fungicida; Microbiologia; Vinho. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/195411/1/ab-gildo.pdf
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Marc: |
LEADER 03362nam a2200265 a 4500
001 1870250
005 2019-10-31
008 2010 bl uuuu u00u1 u #d
100 1 $aSILVA, G. A. da
245 $aCaptan and Folpan inhibition of PCR amplification of 375 bp DNA fragment specific for the 18S region of Saccharomyces.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON CULTURE COLLECTIONS, 12., 2010, Florianópolis. Biological Resource Centrers: gateway to biodiversity and services for innovations in biotechnology: [abstracts]. [S.l]: WFCC$c2010
300 $aNão paginado.
500 $aPoster.
520 $aThe off-flavor metabolites produced by nutritionally fastidious yeasts can be economically disastrous to the wine producer since they render the wine unpalatable, decreasing the quality of the wine, and resulting in a lower sale price. These microorganisms are difficult to be detected by traditional culture media since these techniques involve complex enrichment. The polymerase chain reaction (PCR)is a well established molecular technique that uses tiny amounts of genetic material to be amplified, it does not depend on culture media for cell growth and so it can overcome several difficulties. This essentially enzymatic reaction can be employed to detect microorganisms in foods and beverages but it can also be susceptible to inhibitors. Molecular systematic investigation of members of the ascomycetous yeast genera or monitoring spoilage microorganisms in wine may not be an easy task due the presence of inhibitor compounds. Besides the presence of wine naturally occurring inhibitors, cultural practices, like fungicide treatments can also contribute to add external inhibiting factors if residues of these compounds are present in wine. The inhibition is likely to be dependent upon the residue levels. Captan and Folpan are widely employed in Brazilian vineyards to control fungal threats. The purpose of this work was to detect the action of these compounds upon Taq polymerase using a forward primer Fungf (5'-CTTAGTTGGTGGAGTGATTTG-3') and a reverse primer Fungr (5'-GGTACTAGCGACGGGCG-3') that amplify a 375 bp DNA fragment specific for the 18S region of fungi. The efficiency of PCR and yeast metabolism were evaluated with the strain Saccharomyces cerevisiaeEmbrapa 1vvt/97 (BRM-4687) in the presence of different concentrations of Captan (1200-recommended dose, 600, 300, 150, 75, and 37.5 ?g/mL) and Folpan (1350-recommended dose, 675, 337.5, 168.8, 84.4 and 42.2 ?g/mL). The amplification was observed only at 75, and 37.5 ?g/mL Captan. Negligible amplification was obtained at 84.4 ?g/mL Folpan and visible amplification was possible only at 42.2 ?g/mL Folpan. Captan and Folpan are, depending on the concentration, strong Taq polymerase inhibitors. The presence of both fungicides can make the hard work of monitoring process of spoilage microorganisms in wine even harder. These fungicides also showed to be strong inhibitors of the cell yeast metabolism.Key words:Fermentation, fungicides, PCR, Taq polymerase, yeast
650 $aFermentação
650 $aFungicida
650 $aMicrobiologia
650 $aVinho
653 $aLevedura
653 $aPCR
653 $aPolimerase
700 1 $aBERNARDI, T. L.
700 1 $aSCHAKER, P. D. C.
700 1 $aMELLO, L. M. R. de
700 1 $aVALENTE, P.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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Registro Completo
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Biblioteca(s): |
Embrapa Acre. |
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Data corrente: |
05/03/2001 |
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Data da última atualização: |
09/02/2023 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
SANTOS, J. C. dos; CAMPOS, R. T. |
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Afiliação: |
JAIR CARVALHO DOS SANTOS, CPAF-AC; ROBÉRIO TELMO CAMPOS, UNIVERSIDADE FEDERAL DO CEARÁ. |
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Título: |
Metodologia para análise de rentabilidade e riscos de sistemas agroflorestais. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Rio Branco, AC: Embrapa Acre, 2000. |
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Páginas: |
14 p. |
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Série: |
(Embrapa Acre. Documentos, 47). |
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ISSN: |
0104-9046 |
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Idioma: |
Português |
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Conteúdo: |
Este trabalho propõe uma metodologia para avaliação da rentabilidade e riscos de sistemas agroflorestais e de cada um dos seus componentes culturais, através da combinação da técnica Monte Carlo, comumente utilizada para avaliar riscos em investimentos, com um método de rateio dos custos comuns entre os componentes culturais, possibilitando a análise do sistema como um todo e a análise isolada de cada um desses componentes. |
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Palavras-Chave: |
Acre; Agrofloresta; Agroflorestal; Agroforestry sistem; Agroforestry systems; Agroforetry systems; Agroforrstería; Agroorestry; Análise financeira; Análisis de costo-beneficio; Análisis del riesgo; Aspecto economico; Brasil; Campos; Economic situation; Financier analysis; Método Monte Carlo; Monte Carlo; Probabilidade; Rentabilidad; Rio Branco; Simulation; Sistema agroflorestal; Sistema agroflorestal (SAF); Sistema Agroflorestal - Análise Floresetal; Sistema agroflorestal analise financeira; Species; Sustentability; Tecnica Monte Carlo. |
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Thesagro: |
Agrossilvicultura; Análise; Análise de Custo-Benefício; Análise Econômica; Desenvolvimento Econômico; Desenvolvimento Sustentável; Economia; Espécie; Floresta; Floresta Tropical Úmida; Implantação de Cultura; Método; Pequeno Produtor; Rentabilidade; Risco; Simulação; Uso da Terra. |
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Thesaurus NAL: |
agroforestry; Amazonia; Cost benefit analysis; economic analysis; economic development; economics; land use; Monte Carlo method; Profitability; risk; Risk analysis; small farms; tropical rain forests. |
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Categoria do assunto: |
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A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPAF-AC/3989/1/doc47.pdf
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Marc: |
LEADER 02814nam a2200865 a 4500
001 1495387
005 2023-02-09
008 2000 bl uuuu u0uu1 u #d
022 $a0104-9046
100 1 $aSANTOS, J. C. dos
245 $aMetodologia para análise de rentabilidade e riscos de sistemas agroflorestais.
260 $aRio Branco, AC: Embrapa Acre$c2000
300 $a14 p.
490 $a(Embrapa Acre. Documentos, 47).
520 $aEste trabalho propõe uma metodologia para avaliação da rentabilidade e riscos de sistemas agroflorestais e de cada um dos seus componentes culturais, através da combinação da técnica Monte Carlo, comumente utilizada para avaliar riscos em investimentos, com um método de rateio dos custos comuns entre os componentes culturais, possibilitando a análise do sistema como um todo e a análise isolada de cada um desses componentes.
650 $aagroforestry
650 $aAmazonia
650 $aCost benefit analysis
650 $aeconomic analysis
650 $aeconomic development
650 $aeconomics
650 $aland use
650 $aMonte Carlo method
650 $aProfitability
650 $arisk
650 $aRisk analysis
650 $asmall farms
650 $atropical rain forests
650 $aAgrossilvicultura
650 $aAnálise
650 $aAnálise de Custo-Benefício
650 $aAnálise Econômica
650 $aDesenvolvimento Econômico
650 $aDesenvolvimento Sustentável
650 $aEconomia
650 $aEspécie
650 $aFloresta
650 $aFloresta Tropical Úmida
650 $aImplantação de Cultura
650 $aMétodo
650 $aPequeno Produtor
650 $aRentabilidade
650 $aRisco
650 $aSimulação
650 $aUso da Terra
653 $aAcre
653 $aAgrofloresta
653 $aAgroflorestal
653 $aAgroforestry sistem
653 $aAgroforestry systems
653 $aAgroforetry systems
653 $aAgroforrstería
653 $aAgroorestry
653 $aAnálise financeira
653 $aAnálisis de costo-beneficio
653 $aAnálisis del riesgo
653 $aAspecto economico
653 $aBrasil
653 $aCampos
653 $aEconomic situation
653 $aFinancier analysis
653 $aMétodo Monte Carlo
653 $aMonte Carlo
653 $aProbabilidade
653 $aRentabilidad
653 $aRio Branco
653 $aSimulation
653 $aSistema agroflorestal
653 $aSistema agroflorestal (SAF)
653 $aSistema Agroflorestal - Análise Floresetal
653 $aSistema agroflorestal analise financeira
653 $aSpecies
653 $aSustentability
653 $aTecnica Monte Carlo
700 1 $aCAMPOS, R. T.
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Registro original: |
Embrapa Acre (CPAF-AC) |
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